MiCAN Technologies

Coronavirus optimised myeloid lineage cell - "cMylc"

Exclusively available from Biospire to the global research market

Details

Lab Experiments

OVERVIEW

MiCAN, the world leader of providing stem cell derived myeloid cells launched coronavirus optimised line called "cMylc" to accelerate vaccine and drug discovery research on COVID-19.

Human blood cell based host cells to infect SAR-CoV-2 coronavirus 

Ideal cell tool to evaluate ADE (antibody-dependent enhancement) for safe vaccine development

High reproducible cells using established stem cell technologies

SARS-CoV-2 Infection Data

SARS-CoV-2 virus successfully infected cMylc cells expressing ACE2 and TMPRSS2. cMylc-DC cells, further differentiated using the cMylc-kit are more susceptible to SARS-CoV-2 virus.

Viral RNA detection from supernatant of the infected cell culture. Significant increase of progeny viruses were observed on cMylc-DC with further differentiation.

Viral RNA detection from supernatant of the infected cell culture. Significant increase of progeny viruses were observed on cMylc-DC with further differentiation.

SARS-CoV-2 Infection Data

SARS-CoV-2 virus successfully infected cMylc cells expressing ACE2 and TMPRSS2. cMylc-DC cells, further differentiated using the cMylc-kit are more susceptible to SARS-CoV-2 virus.

Viral RNA detection from supernatant of the infected cell culture. Significant increase of progeny viruses were observed on cMylc-DC with further differentiation.

Viral RNA detection from supernatant of the infected cell culture. Significant increase of progeny viruses were observed on cMylc-DC with further differentiation.

Experimental flow using "cMylc"

1. Preparation of  culture medium

50 uL of supplement is added into 10 mL 10% FBS/aMEM. Remaining supplement could be kept at 4 degC for two weeks (refreezing and thawing not recommended).

2. Preparation of cMylc cells

Cryopreserved cMylc in a frozon vial are thawed rapidly in a 37 degC water bath, and immediately suspended in 10% FBS/aMEM at room temperature. 

[Full protocol is available upon purchasing and request]

3. Cell culture of cMylc

After centrifuging (300G, 5 min at room temperature), cell pellets are resuspended in 10 mL of 10% FBS/aMEM for two days in a humidified atomosphere under 5% CO2 (95% air) at 37 degC.

After two days, cells are centributed at above condition.

 

Cell pellets are resuspended in 10 mL of 10% FBS/aMEM indluding "supplement B" and incubated for 3 days at 37 degC in a low cell binding T-25 flask. cMylc-DC, which is ideal for virus infection, is ready for experiment.

4. Viris infection

cMylc-DC is prepared in a 96 well cell culture plate to be mixed with SARS-CoV-2 viruses. 4 hours after mixing, the inoculum is completely removed from each well, then 10% FBS/aMEM is added. Progeny viruses are detectable in the supernatant after 3 days with quantitative RT-PCR.   

"cMylc" is provided with supplement media as a kit

cMylc cryopreserved cell vial

Storage: Frozen, under -150 degC

Shipping: Frozen, dry-ice

Supplement media A&B for cMylc cells

Storage: Frozen, under -80 degC (recommended)

Shipping: Frozen, dry-ice